RP-HPLC Method Development and Validation for the Estimation of Lornoxicam in Bulk and it’s Solid Dosage Form

The present paper describes simple and sensitive, reversed phase high performance liquid chromatography (RP-HPLC) method has been developed for the determination of lornoxicam in bulk and its tablet dosage form. Chromatographic separation was achieved by using phenomenex C18 column, 250×4.6 mm, 5 μm as stationary phase. Mixture of acetonitrile: phosphate buffer (60:40 v/v) was used as a mobile phase and the pH was adjusted into 7.0 using o-phosphoric acid, at a flow rate of 1.2 ml/min. Lornoxicam standard shows maximum absorption in UV at 390 nm. The method was validated statistically and recovery study was performed as per ICH guidelines. The result of percentage recovery and placebo interference shows that the method was not affected by the presence of excipients which proves suitability of the method and also it was found to be rapid, accurate and